Linear amplification with T7 Drop-seq primers

[Pavel Chubukov]

Hello Drop-seq community!

The first question is when primers with linear amplification will be available?
T7 proved to be superior to STRT, it is known since CEL-seq paper http://www.sciencedirect.com/science/article/pii/S2211124712002288
Followed by MARS-seq.
Of course switch at 5′ may look more user friendly, but the quality of the data is the key for analysis of low expressed genes, like transcription factors:
The actual target of ours in the quest for neuronal diversity.

How do we convince Chemgenes to synthesize beads with T7 promoter at 5′ of the illumina_adaptor_barcode-UMI- ???

Thanks!

[Evan Macosko]

Hi Pavel,

Thanks for your post. It would be interesting to try different amplification strategies with Drop-seq. ChemGenes will make barcoded beads with custom primer sequences. If you do order ones with a T7-compatible sequence and try them, let us know how your libraries look!

Best
Evan

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